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KMID : 0352419920110040561
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Keimyung Medical Journal
1992 Volume.11 No. 4 p.561 ~ p.570
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Effect of Parenteral Administration of Endotoxin on Changes of Serum and Hepatic Carboxylesterase, Arylesterase and Cholinesterase Activities in Rats
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Abstract
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The activities of the cytosolic, microsomal and mitochondrial carboxylesterase, arylesterase and cholinesterase which are detoxifying enzymes in the liver were measured in order to evaluate the detoxifying ability of the liver on parenteral
administration of endotoxin. And the activities of these enzymes in the serum were also measured.
For administration of endotoxin, a dose of 5mg of endotoxin(lipopolysaccharide E. ceoli 026: B6, from Sigma chemical company, USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3, 8
and 24
hours of injection with endooxin to measure the activities of the above enzymes in serum and their liver.
The activities of the cytosolic and mitochondrial esterases in the liver showed no significant changes throughout the experiment. But microsomal carboxylesterase and asrylesterase activities showed a significant decrease between 8 and 24 hours
after
endotoxin administration, and microsomal cholinesterase activity in the liver showed a significant decrease throughout the experiment after endotoxin administration. Serum carboxylesterase and arylesterase activities showed a significant increase
between 8 and 24 hours after endotoxin administration and serum cholinesterase activity showed a signigicant increase at 8 hours after endotoxin administration.
According to the results, the activities of the cytosolic and mitochondrial esterases in the liver showed no significant changes. It is thought that these results are caused by their main location in the endopiasmic reticulum, and caused by
playing
a
trivial role of these enzymes in the detoxification of the endotoxin. And decreased microsomal esterases activities in the liver are thought due to decreased systhesis of there enzymes in the microsome and due to leak into the blood throught the
damaged
membrane of hepatocyte. The activities of these esterases in the liver is decreased. So it is thought that the detoxifying enzymes in the liver play only a trivial role in the detoxification of the endotoxin.
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KEYWORD
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